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¡ì Immune Cell Phenotyping and Functions µ¹¾Æ°¡±â


iQue¢ç Human General Immune Cell Killing Kit ´Â ¼¼Æ÷ÀÇ phenotyping °ú ±â´ÉÀû marker ¸¦ È®ÀÎÇÒ ¼ö ÀÖÀ¸¸ç ¶ÇÇÑ bead ±â¹ÝÀÇ ±â¹ýÀ» ÀÌ¿ëÇÏ¿© ºÐºñµÈ cytokine À» ÃøÁ¤ÇÒ ¼ö ÀÖ½À´Ï´Ù. washing ´Ü°è°¡ ¾ø¾î ½ÇÇè°úÁ¤ÀÌ ¸Å¿ì Æí¸®ÇÏ¸ç ¶ÇÇÑ ¼¼Æ÷¿Í ºñµå¸¦ µ¿½Ã¿¡ ÃøÁ¤°¡´ÉÇÏ¿© ´ÙÀ½°ú °°Àº ½ÇÇèÀû ÀÌÇظ¦ ¸¹Àº µµ¿òÀ» ÁÙ ¼ö ÀÖ½À´Ï´Ù.
fluorescent membrane integrity dye ¿Í mitochondrial membrane potential dye ¸¦ ÀÌ¿ëÇÏ¿© »ì¾ÆÀÖ´Â ¸é¿ª¼¼Æ÷¿Í Á×Àº ¼¼Æ÷¸¦ ±¸º°ÇÒ ¼ö ÀÖ½À´Ï´Ù.
fluorescent encoder dye ¸¦ ÀÌ¿ëÇÏ¿© ¼¼Æ÷¸¦ ¿°»öÇÏ°Ô µÇ¸é Target ¼¼Æ÷¿Í Effector ¼¼Æ÷¸¦ ±¸º° ÇÒ ¼ö ÀÖ½À´Ï´Ù.
mitochondrial membrane depolarization, ¼¼Æ÷ÀÇ membrane integrity ¼Ò½Ç, cell count, ±×¸®°í pro-apoptotic protease Granzyme B ¸¦ Æ÷ÇÔÇÏ´Â ÇϳªÀÇ assay ¸¦ ÅëÇØ ¸é¿ª¼¼Æ÷¿¡ ÀÇÇØ ÁøÇàµÇ´Â Cell Killing ÀÇ º¹ÀâÇÑ ¸ÞÄ¿´ÏÁòÀ» µ¿½Ã ºÐ¼®ÀÌ °¡´ÉÇÕ´Ï´Ù.


Key Benefits
¡î Speed : ºü¸¥ ½Ã·á 󸮷κÎÅÍ ½ÇÁúÀûÀÎ µ¥ÀÌÅ͸¦ ¾òÀ½À¸·Î½á ÇÑ ¹ø¿¡ ¿©·¯ Á¾·ùÀÇ ½ÇÇèÀ» µ¿½Ã¿¡ ½ÇÇàÇÏ¿©, ¼ÕÀ¸·Î Á÷Á¢ ½ÇÇèÇÏ´Â ½Ã°£°ú ÃÑ ½ÇÇèȽ¼ö¸¦ ÁÙÀÌ°í, ¼ÒÁßÇÑ ½Ã·á¸¦ º¸Á¸ÇÏ´Â °ÍÀÌ °¡´ÉÇÕ´Ï´Ù
¡î Results : single well assay ¸¦ ÅëÇØ target and effector cells, mitochondrial membrane depolarization, cell membrane integrity, cell count, ±×¸®°í ºÐºñµÈ pro-apoptotic protease Granzyme B ÀÇ ÃøÁ¤ÀÌ °¡´ÉÇÕ´Ï´Ù.
¡î Biological insights : ÇϳªÀÇ plate well ¿¡¼­ ¸é¿ª¼¼Æ÷¿¡ ÀÇÇØ ÁøÇàµÇ´Â º¹ÀâÇÑ Cell Killing ¸ÞÄ«´ÏÁòÀ» Á¶»çÇÒ ¼ö ÀÖ½À´Ï´Ù.
¡î Ease of use : ÇϳªÀÇ platform ¿¡¼­ assay ÁøÇàÀ» ÇÏ°í ¶ÇÇÑ °°Àº platform ¿¡ ÀÖ´Â iQue Forecyt¢ç ¼ÒÇÁÆ®¿þ¾î¸¦ ÀÌ¿ëÇÏ¿© µ¥ÀÌÅ͸¦ ºÐ¼®ÇϹǷΠ¿©·¯ ¼Ò½º·Î ºÎÅÍ µ¥ÀÌÅ͸¦ ºÒ·¯¿À°í ºñ±³ÇÏ´Â º¹ÀâÇÑ °úÁ¤ÀÌ ¾øÀ¸¹Ç·Î ½Ã°£ÀÌ ¸Å¿ì Àý¾àµÇ°í Æí¸®ÇÕ´Ï´Ù.



mitochondrial membrane depolarization, loss of cell membrane integrity, cell count, ±×¸®°í pro-apoptotic protease ¸¦ Æ÷ÇÔÇÏ´Â ¸é¿ª¼¼Æ÷¿¡ ÀÇÇØ ÁøÇàµÇ´Â º¹ÀâÇÑ Cell Killing ¸ÞÄ«´ÏÁòÀ» µ¿½Ã¿¡ ºÐ¼®ÇÒ ¼ö ÀÖ½À´Ï´Ù.
¹ÌÅäÄܵ帮¾Æ ¿Ü¸·ÀÇ ÀüÀ§Â÷´Â MOMP (Mitochondrial outer membrane potential) ·Îµµ ¾Ë·ÁÁ® ÀÖ½À´Ï´Ù.
Target Cell Àº fluorescent encoding dye ¸¦ ÀÌ¿ëÇÏ¿© Effector Cell °ú ±¸º°ÀÌ µÇ¾îÁý´Ï´Ù.



Workflow of iQue¢ç Human General Immune Cell Killing Kit



 
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